Figure 2. Initial analysis of vinyl nucleoside incorporation into RNA.
A. Crystal structures of uridine-cytidine kinase and adenosine kinase bound to cytidine and adenosine, respectively. PDB: 1UEI, 1BX4 B. Structures of vinyl-nucleosides C. Incorporation levels of vinyl-nucleosides. HEK293T cells were treated for 12 h at 1 mM final concentration, after which RNA was isolated. Percent incorporation was determined through total RNA digestion and LC-MS/MS analysis of mono-nucleoside mixtures.