Table 4. In vitro digestibility using the Daisy^II Incubator and according to the method described in Hervera et al. [38], (HV-IVD) and in Biagi et al. [39], (BG-IVD).

Step	HV-IVD	BG-IVD
	0.5 ± 0.01 g of sample1	0.5 ± 0.01 g of sample
1 Gastric digestion	1200 ml phosphate buffer (0.1M, pH 6) 480 ml HCl (0.2M) 480 mg pepsin 24 ml cloramphenicol solution (0.5g/100 ml ethanol) pH 2, 39°C, 2 h	1440 ml of pepsin-lipase-HCl solution (HCl 0.075N; pepsin 2g/L; gastric lipase 1g/L) 39°C, 2 h
2 Post-gastric digestion	480 ml phosphate buffer (0.2 M, pH 6.8) 240 ml NaOH (0.6 M) 4.8 g pancreatin pH 6.8, 39°C, 4 h	1440 ml -pancreatin-bile salt-phosphate buffer solution (10g/L pancreatin 25 g/L; bile salt) pH 7.5, 39°C, 4 h
3 Collection of undigested fraction	F57 washed, twiced with ethanol (96%) and twice with acetone (99%), dried overnight at 70°C, analysed for CP, EE, DM, OM	F57 washed, dried overnight at 65°C, analysed for CP, EE, DM, OM

¹ Quantities for each jar and twenty-four F57 filter bags (twenty-three replicate samples and one blank).