Skip to main content
. 2021 Apr 22;16(4):e0249988. doi: 10.1371/journal.pone.0249988

Fig 1. Orbital fibroblasts (OFs) from TAO patients secreted higher levels of IGF-1 and expressed higher levels of IGF-1 mRNA.

Fig 1

(A) OFs from control individuals (#71, #116, #136) and patients with TAO (#120, #135, #137) were plated at a concentration of 2 x 105 cells/well in a 6-well plate. After 24 hours, the culture medium was analyzed for IGF-1 content using an ELISA kit (R&D Systems, Minneapolis, MN). (B) IGF-1 mRNA expression was determined by quantitative real time-RT–PCR.