Fig 4. OFs from patients with TAO showed higher levels of NF-κB activity.
(A) OFs were plated at a concentration of 2 x 105 cells/well in a 6-well plate. After 24 hours, NF-κB activity in OFs from control individuals (#71, #116, #136) and patients with TAO (#120, #135, #137) was determined by measuring the levels of degradation of IκB and phosphorylation of IKKα/β and NF-κB p65 via a Western blot analysis. (B) OFs from control individuals (#111, #152, #155, 158) and patients with TAO (#107, #110, #135, #162) were co-transfected with pGL4.32[luc2P/NF-κB-RE/Hygro] for firefly luciferase and pRL-TK for renilla luciferase. After 24 hours, cell extracts were assayed for firefly and renilla luciferase activity. The results are expressed as the ratio of firefly-to-renilla luciferase activity (Fluc/Rluc).