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. 2021 Apr 12;17(4):e1009498. doi: 10.1371/journal.pgen.1009498

Fig 1. DNA methylation increases during differentiation.

Fig 1

(A) Schematic representation of the ChIP-BS-seq-based analysis of mouse ESCs and differentiated cells (Diff). (B) Quality of BS-seq was analyzed by spiking in oligonucleotides with 5mC and 5C. Plotted is accuracy (%) for each nucleotide. (C) Upper panel: Bar plot of percentages of C, 5mC, mixed (in which adjacent CpGs had different methylation states), and non-CpG reads for ChIP experiments and input for ESCs and differentiated cells. Lower panel: Log2 fold change between C and 5mC reads for each condition. (D) Percentage of 5mC in ChIP experiments and input in ESCs and differentiated cells.