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. Author manuscript; available in PMC: 2021 Dec 23.
Published in final edited form as: J Am Chem Soc. 2020 Dec 14;142(51):21249–21253. doi: 10.1021/jacs.0c09841

Figure 3.

Figure 3.

Distributions of protein-RNA binding kinetics. (a) On-rate distribution for eIF4E binding to 322 arbitrarily-chosen mRNAs from the surface-immobilized population. (b) eIF4E-mRNA on-rate distribution in the presence of eIF4A. (c) Schematic with relative mRNA-binding sites of eIF4E and eIF4A. (d) Distribution of eIF4E-mRNA event durations in the absence (blue) and presence (green) of eIF4A. (e) Correlation of eIF4E-mRNA association rate with the extent of cap-proximal 20 nucleotides, as measured by PARS score. (f) Correlation of eIF4E-mRNA association rate with computed folding free energy change at 30˚C for the cap-proximal 20 nucleotides.