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. 2021 Apr 22;10:e58686. doi: 10.7554/eLife.58686

Figure 5. Plasmatocyte subpopulations are sparse in white pre-pupae.

Figure 5.

(a–b) Dorsal and ventral views of negative control (a, UAS-stinger, but no driver) and positive control (b, UAS-stinger driven by srpHemo-AD;srpHemo-DBD) white pre-pupae (WPP); boxes indicate regions shown in close-up views of positive controls. (c–f) dorsal and ventral views of WPP containing cells labelled using srpHemo-AD and the indicated VT-DBD to drive expression from UAS-stinger. Very few VT enhancer-labelled cells can be detected in WPP: boxes mark regions shown in close-up views with example hemocytes indicated with an arrow; dorsal vessel-associated and proventricular region/Garland cells can also be observed in VT32897 WPP (arrowheads in d and d’, respectively); scale bars represent 500 μm (WPP) or 50 μm (close-ups); WPP images contrast enhanced to 0.3% saturation; close-up images contrast enhanced individually. See Supplementary file 1 for full list of genotypes.