Video 4. Localisation and dynamics of subpopulation cells within L3 larvae.

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Movies showing localisation and movement of cells labelled using split GAL4 lines (srpHemo-AD in combination with VT17559-DBD, VT32897-DBD, VT57089-DBD, or VT62766-DBD) to drive expression from UAS-stinger in L3 larvae. Images taken from timelapse series of single focal planes to enable rapid imaging. Far fewer cells are visible compared to L1 and L2 larvae and the majority of cells detected appear attached to the body wall, since they do not shift their relative positions during larval movements. Movies repeat with second repetition showing examples of rare cells in circulation (illustrated by overlaid tracks). Scale bars represent 500 μm. See Supplementary file 1 for full list of genotypes.