| Gene (Drosophila melanogaster) |
w |
NA |
FLYB:FBgn0003996 |
NA |
| Gene (D. melanogaster) |
srp |
NA |
FLYB:FBgn0003507 |
NA |
| Gene (D. melanogaster) |
crq |
NA |
FLYB:FBgn0015924 |
NA |
| Gene (D. melanogaster) |
simu |
NA |
FLYB:FBgn0260011 |
Also known as NimC4
|
| Gene (D. melanogaster) |
eater |
NA |
FLYB:FBgn0243514 |
NA |
| Gene (D. melanogaster) |
Cnx14D |
NA |
FLYB:FBgn0264077 |
NA |
| Gene (D. melanogaster) |
repo |
NA |
FLYB:FBgn0011701 |
NA |
| Genetic reagent (D. melanogaster) |
w1118 |
Evans lab stock |
FLYB:FBal0018186 |
FlyBase symbol:w1118
|
| Genetic reagent (D. melanogaster) |
srpHemo-GAL4 |
PMID:15239955
|
FLYB:FBtp0023390 |
FlyBase symbol:P{srp.Hemo-GAL4}2; Obtained from W. Wood, University of Edinburgh, UK |
| Genetic reagent (D. melanogaster) |
srpHemo-Gal4,UAS-GFP |
PMID:16651377
|
FLYB:FBtp0023390 (P{srp.Hemo-GAL4}2) |
Obtained from W. Wood, University of Edinburgh, UK |
| Genetic reagent (D. melanogaster) |
srpHemo-GAL4,UAS-red stinger |
PMID:23172914
|
FLYB:FBtp0023390 (P{srp.Hemo-GAL4}2) |
Obtained from B. Stramer, Kings College London, UK |
| Genetic reagent (D. melanogaster) |
crq-GAL4,UAS-GFP |
PMID:15699212
|
FLYB:FBtp0022491 (P{crq-GAL4.A}) |
Obtained from W. Wood, University of Edinburgh, UK |
| Genetic reagent (D. melanogaster) |
da-GAL4 |
PMID:FBrf0082789 |
FLYB:FBtp0019571 |
FlyBase symbol:P{da-GAL4.w-}; Obtained from A. Whitworth, University of Cambridge, UK |
| Genetic reagent (D. melanogaster) |
UAS-GC3ai |
PMID:28870988
|
FLYB:FBtp0137390 |
FlyBase symbol:P{UAS-GC3Ai}3; Obtained from M. Suzanne, CBI-Toulouse, France |
| Genetic reagent (D. melanogaster) |
srpHemo-3x-mCherry |
Bloomington Drosophila Stock Center; PMID:29321168
|
BDSC: 78359; FLYB:FBtp0127793; RRID:BDSC_ 78359 |
FlyBase symbol:P{srpHemo-3XmCherry}; Obtained from B, Stramer, Kings College London, UK |
| Genetic reagent (D. melanogaster) |
Hml(Δ)-GAL4,UAS-GFP |
Bloomington Drosophila Stock Center; PMID:15480416
|
BDSC:30140; RRID:BDSC_30140; FLYB:FBtp0040877; BDSC:30142; RRID:BDSC_30142; FLYB:FBtp0040877 |
FlyBase symbol:‘w1118; P{Hml-GAL4.Δ}2, P{UAS-2xEGFP}AH2’;‘w1118; P{Hml-GAL4.Δ}3, P{UAS-2xEGFP}AH3/MKRS’ |
| Genetic reagent (D. melanogaster) |
VT17559-GAL4 |
PMID:24896182
|
VDRC:205658 |
Previously available from Vienna Drosophila Research Center (stock discarded); available on request from I. Evans |
| Genetic reagent (D. melanogaster) |
VT32897-GAL4 |
PMID:24896182
|
VDRC:214064 |
Previously available from Vienna Drosophila Research Center (stock discarded); available on request from I. Evans |
| Genetic reagent (D. melanogaster) |
VT57089-GAL4 |
PMID:24896182
|
VDRC:208119 |
Previously available from Vienna Drosophila Research Center (stock discarded); available on request from I. Evans |
| Genetic reagent (D. melanogaster) |
VT62766-GAL4 |
PMID:24896182
|
VDRC:203897 |
Previously available from Vienna Drosophila Research Center (stock discarded); available on request from I. Evans |
| Genetic reagent (D. melanogaster) |
UAS-tdTomato |
Bloomington Drosophila Stock Center |
BDSC:36327; FLYB:FBti0145103; RRID:BDSC_36327
|
FlyBase symbol:P{UAS-tdTom.S}2 |
| Genetic reagent (D. melanogaster) |
srpHemo-GMA |
Other |
NA |
Globular Moesin actin-binding domain fused to GFP under the control of srpHemo; P-element insertions on chromosomes 2 and 3; Obtained from James Bloor, University of Kent, UK |
| Genetic reagent (D. melanogaster) |
UAS-GFP |
Bloomington Drosophila Stock Center |
BDSC: 5431; FLYB: FBti0013988; RRID:BDSC_5431
|
FlyBase symbol:P{UAS-EGFP}5a.2 |
| Genetic reagent (D. melanogaster) |
eater-GFP |
PMID:17936744
|
FLYB: FBtp0054463 |
FlyBase symbol:P{eater-GFP.1.7}; Obtained from L. Vesala, University of Tampere, Finland |
| Genetic reagent (D. melanogaster) |
simu-cytGFP |
PMID:18455990
|
NA |
FlyBase symbol:M{simu-cytGFP}; Obtained from E. Kurant, University of Haifa, Israel |
| Genetic reagent (D. melanogaster) |
VT17559-RFP |
This paper |
NA |
Inserted in attP2 on chromosome 3; see methods for details of cloning and transgenesis |
| Genetic reagent (D. melanogaster) |
VT32897-RFP |
This paper |
NA |
Inserted in attP2 on chromosome 3; see methods for details of cloning and transgenesis |
| Genetic reagent (D. melanogaster) |
VT57089-RFP |
This paper |
NA |
Inserted in attP2 on chromosome 3; see methods for details of cloning and transgenesis |
| Genetic reagent (D. melanogaster) |
VT62766-RFP |
This paper |
NA |
Inserted in attP2 on chromosome 3; see methods for details of cloning and transgenesis |
| Genetic reagent (D. melanogaster) |
srpHemo-AD |
This paper |
NA |
Inserted in VK1 attP site on; see methods for details of cloning and transgenesis chromosome 3 |
| Genetic reagent (D. melanogaster) |
srpHemo-DBD |
This paper |
NA |
Inserted in attP2 on chromosome 3; see methods for details of cloning and transgenesis |
| Genetic reagent (D. melanogaster) |
VT17559-DBD |
This paper |
NA |
Inserted in attP2 on chromosome 3; see methods for details of cloning and transgenesis |
| Genetic reagent (D. melanogaster) |
VT32897-DBD |
This paper |
NA |
Inserted in attP2 on chromosome 3; see methods for details of cloning and transgenesis |
| Genetic reagent (D. melanogaster) |
VT57089-DBD |
This paper |
NA |
Inserted in attP2 on chromosome 3; see methods for details of cloning and transgenesis |
| Genetic reagent (D. melanogaster) |
VT62766-DBD |
This paper |
NA |
Inserted in attP2 on chromosome 3; see methods for details of cloning and transgenesis |
| Genetic reagent (D. melanogaster) |
srpHemo-H2A-3x-mCHerry |
Bloomington Drosophila Stock Center; PMID:29321168
|
BDSC: 78361; FLYB:FBtp0127794; RRID:BDSC_78661
|
FlyBase symbol:P{srpHemo-H2A.3XmCherry}; Obtained from B. Stramer, Kings College London, UK |
| Genetic reagent (D. melanogaster) |
UAS-stinger |
Bloomington Drosophila Stock Center |
BDSC:84277; FLYB:FBti0074589; RRID:BDSC_84277
|
FlyBase symbol:P{UAS-Stinger}2 |
| Genetic reagent (D. melanogaster) |
‘w;;UAS-red stinger,UAS-FLP,Ubi-p63E(FRT.STOP)Stinger’ |
Bloomington Drosophila Stock Center; PMID:19633663
|
BDSC:28281; RRID:BDSC_28281
|
FlyBase symbol:‘w[*]; P{w[+mC]=UAS-RedStinger}6, P{w[+mC]=UAS-FLP.Exel}3, P{w[+mC]=Ubi-p63E(FRT.STOP)Stinger}15F2’; Obtained from Alisson Gontijo, CEDOC, Lisbon, Portugal |
| Genetic reagent (D. melanogaster) |
UAS-tdTomato |
Bloomington Drosophila Stock Center |
BDSC:36327; FLYB:FBti0145103; RRID:BDSC_36327
|
FlyBase symbol:P{UAS-tdTom.S}2 |
| Genetic reagent (D. melanogaster) |
UAS-GFP-myc-2xFYVE |
Bloomington Drosophila Stock Center |
BDSC:42712; FLYB:FBti0147756; RRID:BDSC_42712
|
FlyBase symbol:P{UAS-GFP-myc-2xFYVE}2 |
| Genetic reagent (D. melanogaster) |
UAS-Cnx14D |
Harvard Drosophila Stock Center |
FLYB:FBal0228355 |
FlyBase symbol:P{XP}parad04188; Previously available from Harvard Drosophila Stock Centre (now discarded); available on request from I. Evans |
| Genetic reagent (D. melanogaster) |
repo03702 |
Bloomington Drosophila Stock Center; PMID:32796812
|
BDSC:11604; FLYB:FBti0003552; RRID:BDSC_11604
|
FlyBase symbol:ry506 P{PZ}repo03702/TM3, ryRK Sb1 Ser1; ry506 allele recombined off original stock line |
| Biological sample (D. melanogaster) |
Embryos, L1-L3 larvae, white pre-pupae, pupae, adults, hemolymph (larval and adult) |
NA |
NA |
NA |
| Biological sample (S. cerevisiae) |
Isogenic S. cerevisiae wild-type yeast strain BY4741 |
Euroscarf consortium |
Euroscarf: Y00000 |
Heat-killed and stained with calcofluor staining solution for use in phagocytosis assay; 1 × 106 particles added per well in 96-well plate |
| Antibody |
Anti-cleaved DCP-1 (Asp216) (Rabbit polyclonal) |
Cell Signaling Technologies |
Cat# 9578S; RRID:AB_2721060
|
IF(1:1000); Primary antibody used to detect apoptotic cells |
| Antibody |
Anti-GFP (Mouse monoclonal) |
Abcam |
Cat# ab1218; RRID:AB_298911
|
IF(1:200); Primary antibody used to detect cells expressing GFP |
| Antibody |
Alexa Fluor 488 Goat anti-Mouse IgG (Goat polyclonal) |
Invitrogen/Molecular Probes |
Cat# A11029; RRID:AB_138404
|
IF(1:400); secondary antibody used to detect anti-GFP primary antibody |
| Antibody |
Alexa Fluor 568 Goat anti-Rabbit IgG (Goat polyclonal) |
Invitrogen/Molecular Probes |
Cat# A11036; RRID:AB_10563566
|
IF(1:400); secondary antibody used to detect anti-cleaved DCP-1 primary antibody |
| Software, algorithm |
Fiji |
PMID:22743772
|
RRID:SCR_002285
|
|
| Software, algorithm |
GraphPad Prism 7 |
Graphpad |
RRID:SCR_002798
|
|
| Other |
NucBlue |
Invitrogen/Molecular Probes |
Cat# R37605
|
NucBlue Live ReadyProbes Reagent (Hoechst 33342); two drops per ml; nuclear stain |
| Other |
Calcofluor staining solution |
Sigma-Aldrich |
Cat# 18909–100 ML-F |
40 μl used to stain 1 × 109 heat-killed yeast particles in 1 mL PBS |
| Other |
Dihydrorhodamine 123 ROS dye |
Sigma-Aldrich |
Cat# D1054-10MG |
ROS dye; used at (50 μM) in PBS to stain embryos |
| Other |
pHrodo green E. coli BioParticles |
Invitrogen/Molecular Probes |
Cat# P35366
|
Microinjected into embryos at (1 mg/ml) |
| Other |
Alexa Fluor 647 phalloidin |
Invitrogen/Molecular Probes |
Cat# A22287; RRID:AB_2620155
|
1:200 Dilution |
| Other |
Alexa Fluor 568 phalloidin |
Invitrogen/Molecular Probes |
Cat# A12379 |
1:500 Dilution |
