Figure 6. Effect of O. formigenes colonization on fecal and urinary oxalate.
(A) Study design of the mouse experiment. C57Bl/6 mice from our gnotobiotic facility were assigned to three groups. At days 0, 2, and 7, mice were gavaged (blue arrowheads) with O. formigenes strain OXCC13 (n = 5), O. formigenes freshly isolated from a primary hyperoxaluria type 1 (PH1) subject (n = 6), or O. formigenes culture medium alone (Media). Mice were fed with normal chow supplemented with 1% sodium oxalate and 0.5% calcium from day −5 until sacrifice. Urine was obtained from a 48 hr collection (one to two mice per pool) prior to sacrifice, and feces were collected at sacrifice (blue arrowhead). (B, C) Urinary and fecal oxalate in three mouse groups. Urinary oxalate normalized by creatinine in the 48 hr urine samples and fecal oxalate levels per gram of stool samples in the three experimental groups. *p<0.05, by Tukey’s multiple comparisons test. (D) Relationship between fecal and urinary oxalate. Correlation coefficient was computed using Spearman’s r.
Figure 6—figure supplement 1. Detection of Oxalobacter formigenes by qPCR in the mouse fecal samples before sacrifice.
Figure 6—figure supplement 2. Detection of O. formigenes by qPCR in the intestinal contents (Jej: jejenum, Ile: ileum) and mouse fecal (Fec) samples at sacrifice.
