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. 2021 Apr 15;2021:7848027. doi: 10.1155/2021/7848027

Figure 4.

Figure 4

Rap1GAP knockdown attenuates cardiomyocyte hypertrophy and enhanced Ang II-induced autophagy. (a) mRNA levels of Rap1GAP, ANF, and BNP in Rap1GAP knockdown and intact cardiomyocytes determined using RT-PCR. (b) Western blot analysis for LC3BII/I, p62, and cleaved-caspase-3/caspase-3 in Rap1GAP-deficient cardiomyocytes. (c) Representative images and the quantifications from α-actinin staining showing cardiomyocyte surface area in Rap1GAP-deficient cardiomyocytes. Scale bar: 20 μm; n = 10. (d) Representative images from a fluorescence microscope showing Rap1GAP knockdown vs. intact NRCMs infected with adenovirus mRFP-GFP-LC3. Scale bar: 20 μm. (e) Representative images and quantifications from TEM showing autophagy lysosomes, autophagosomes, and mitochondria. The red arrows indicating autophagy lysosome or autophagosome and the yellow boxes indicating mitochondria. Scale bar: 1.0 μm. ∗P < 0.05 and ∗∗P < 0.01. Results are the mean ± SEM of three independent experiments (n = 3).