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. 2021 Apr 2;6(1):bpab007. doi: 10.1093/biomethods/bpab007

Figure 2:

Figure 2:

Analysis of PCR amplified fragments by agarose gel electrophoresis. (A) Lanes 1 and 12—GeneRuler ULR DNA Ladder (Thermo Scientific). Lanes 2–11—amplified products of each of the 10 fragments carrying the intended mutations after the first PCR (B) Lane1—GeneRuler 1 kb ladder (Thermo Scientific); Lanes 2 and 3—final joined fragments after the second PCR. (C) Lane1—GeneRuler 1 kb ladder; Lanes 2–10—PCR amplified products confirming the presence of the whole construct from transformant E. coli colonies.