Table 1:
Reagent | Amount/reaction (µl) |
---|---|
Q5 PCR buffer | 10 |
10 mM dNTP | 1 |
Q5 DNA polymerase | 0.5 |
10 µM forward primer | 2.5 |
10 µM reverse primer | 2.5 |
Template | 1 |
Molecular grade water | 32.5 |
Total | 50 |
Reagent | Amount/reaction (µl) |
---|---|
Q5 PCR buffer | 10 |
10 mM dNTP | 1 |
Q5 DNA polymerase | 0.5 |
10 µM forward primer | 2.5 |
10 µM reverse primer | 2.5 |
Template | 1 |
Molecular grade water | 32.5 |
Total | 50 |