Table 2:
Fragments and their sizes
| Serial no. | Fragments generated by primer combinations | Sizes (bp) |
|---|---|---|
| Fragment 1 | Promoter region start to amino acid residue 21 (TEM-1_for+L21F_rev) | 161 |
| Fragment 2 | Residues 21–39 (L21F_for+Q39K_rev) | 81 |
| Fragment 3 | Residues 39–69 (Q39K_for+M69I_rev) | 116 |
| Fragment 4 | Residues 69–104 | 131 |
| (M69I_for+E104K_rev) | ||
| Fragment 5 | Residues 104–164 (E104K_for+R164C/S_rev) | 204 |
| Fragment 6 | Residues 164–182 | 80 |
| (R164C/S_for+M182T_rev) | ||
| Fragment 7 | Residues 182–244 | 209 |
| (M182T_for+Mut238240244_rev) | ||
| Fragment 8 | Residues 244–265 | 101 |
| (Mut238240244_for+T265M_rev) | ||
| Fragment 9 | Residues 265–275 | 61 |
| (T265M_for+R275L/Q_rev) | ||
| Fragment 10 | Residue 275 to end of TEM-1 gene segment (R275L/Q_for+TEM-1_rev) | 61 |
The primer combinations used to generate the fragments are shown inside the parentheses.