Table 3:
sequences of primers used with the mutated bases shown in bold
| Primer name | Primer sequence 5′–3′ |
|---|---|
| TEM-1_for | ACGGAATTCCGCGGAACCCCTATTTGTTTATTTTTC |
| TEM-1_rev | ACGAAGCTTCCAATGCTTAATCAGTGAGGCAC |
| L21F_for | GCGGCATTTTGCTTTCCTGTTTTTGCT |
| L21F_rev | AGCAAAAACAGGAAAGCAAAATGCCGC |
| Q39K_for | GATGCTGAAGATAAGTTGGGTGCACGA |
| Q39K_rev | TCGTGCACCCAACTTATCTTCAGCATC |
| M69I_for | CGTTTTCCAATGATCAGCACTTTTAAA |
| M69I_rev | TTTAAAAGTGCTGATCATTGGAAAACG |
| E104K_for | AATGACTTGGTTAAGTACTCACCAGTC |
| E104K_rev | GACTGGTGAGTACTTAACCAAGTCATT |
| R164C/S_for | ACTCGCCTTGATWGTTGGGAACCGGAG |
| R164C/S_rev | CTCCGGTTCCCAACWATCAAGGCGAGT |
| M182T_for | CGTGACACCACGACGCCTGTAGCAATG |
| M182T_rev | CATTGCTACAGGCGTCGTGGTGTCACG |
| Mut238240244_for | AAATCTGGAGCCAGTAAGCGTGGGTCTHGCGGTATCATTGCA |
| Mut238240244_rev | TGCAATGATACCGCDAGACCCACGCTTACTGGCTCCAGATTT |
| T265M_for | GTAGTTATCTACATGACGGGGAGTCAG |
| T265M_rev | CTGACTCCCCGTCATGTAGATAACTAC |
| R275L/Q_for | ACTATGGATGAACDAAATAGACAGATCGCT |
| R275L/Q_rev | AGCGATCTGTCTATTTHGTTCATCCATAGT |
Degenerate bases: W = A or T; D = A or G or T; H = A or C or T, Mut238240244_for and Mut238240244_rev primers denote mutations of three amino acid residues, namely, G238S, E240K, and R244C/S.