Figure 4.
Schematic diagram of chemiluminescence detection-high performance liquid chromatography (CL-HPLC) for the lipid hydroperoxides assay. A, mobile phase; P, pump; I, sample injection valve; S, sample; C, column in a column oven; U, UV detector; J, mixing joint; B, chemiluminescence reagent consisting of cytochrome c and luminol in borate buffer; Q, spiral Teflon cell; PM, photomultiplier; CL, chemiluminescence detector; R, multiple recorder and integrator; W, waste. Adapted with permission from Ref. 26. Copyright 1990 John Wiley and Sons.