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. 2021 Apr 9;97(4):161–196. doi: 10.2183/pjab.97.010

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© 2021 The Japan Academy

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 20. — Diastereomer analysis of phosphatidylcholine hydroperoxide (PCOOH) bearing 13-9Z,11E-HPODE. PCOOH, prepared via lipoxygenase (LOX)-catalyzed oxidation or auto-oxidation, was subjected to stereoselective liquid chromatography ultraviolet mass spectrometry (LC-UV-MS) to evaluate the R/S configuration of the hydroperoxy group in the PCOOH molecule. PCOOH was detected by UV (234 nm; A) and structure-selective selected reaction monitoring (m/z 812.5/724.4; B). In addition, we prepared PCOOH by directly treating phosphatidylcholine (1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine, 16 : 0/18 : 2 PC) with LOX. The resultant PCOOH was analyzed by stereoselective LC-UV-MS. Adapted with permission from Ref. 98. Copyright 2015 Elsevier.