. 2021 Apr 9;97(4):161–196. doi: 10.2183/pjab.97.010

Table 5.

Optimal oxidation procedure to prepare lipid hydroperoxide (LOOH)

Lipid		Oxidation procedure	Time	Temp	Yielded LOOH^a
	mg		h	℃		mg
PLPC	100	RB-photo	8	4	PLPCOOH	30
	100	UV-photo	24	20	PLPCOOH	1.7
	100	LOX-1	12	40	PLPCOOH	96
PLPE	100	RB-photo	10	4	PLPEOOH	28
	100	UV-photo	24	20	PLPEOOH	1.4
	100	LOX-1	12	40	PLPEOOH	92
PLPS	100	RB-photo	10	4	PLPSOOH	30
	100	UV-photo	24	20	PLPSOOH	1.3
	100	LOX-1	12	40	PLPSOOH	94
ChL	100	RB-photo	8	4	ChLOOH	27
	100	UV-photo	24	20	ChLOOH	1.1
	100	LOX-1	24	40	ChLOOH	6.2
LLL	100	RB-photo	6	4	LLLOOH	31
	100	UV-photo	6	20	LLLOOH	35
	100	LOX-1	12	40	LLLOOH	9.4
LA	100	RB-photo	8	4	LAOOH	32
	100	UV-photo	12	20	LAOOH	35
	100	LOX-1	6	20	LAOOH	99
LAMe	100	RB-photo	8	4	LAMeOOH	34
	100	UV-photo	12	20	LAMeOOH	36
	100	LOX-1	8	20	LAMeOOH	97

LOX-1, lipoxygenase-1; PLPC, 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine; PLPCOOH, 1-palmitoyl-2-hydroperoxyoctadecadienoyl-sn-glycero-3-phosphocholine; PLPE, 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphoethanolamine; RB, Rose Bengal; UV, ultraviolet.

^aLC retention times and mass spectrometry (MS) profiles of hydroperoxides are as follows; PLPCOOH, 11.0 min, 790.6 [M + H]⁺; PLPEOOH, 10.5 min, 748.5 [M + H]⁺; PLPSOOH, 8.5 min, 790.4 [M − H]⁻; ChLOOH, 26.5 min, 703.6 [M + Na]⁺; LLLOOH, 23.0 min, 933.7 [M + Na]⁺; LAOOH, 15.5 min, 330.3 [M + NH₄]⁺; LAMeOOH, 11.5 min, 349.3 [M + Na]⁺.