. 2021 Apr 9;97(4):161–196. doi: 10.2183/pjab.97.010

Table 7.

Optimal conditions for LOOH regeneration from perketal

Isolated perketal		Added PPTS	Total reaction volume	Time	Temp	Yielded LOOH^b		LOOH after LC isolation	Purity of isolated LOOH
in 25 mL of chloroform/methanol (1 : 1)		mg/5 mL of chloroform/methanol (1 : 1)	mL	h	℃		mg	mg	%
PLPCOOMxP	(RB-photo,^a 28 mg)	10	30	6	4	PLPCOOH	27	16	98
	(UV-photo, 1.5 mg)	10	30	2	4	PLPCOOH	1.4	0.9	95
	(LOX-1, 92 mg)	10	30	12	4	PLPCOOH	90	75	>99
PLPEOOMxP	(RB-photo, 22 mg)	10	30	6	4	PLPEOOH	20	12	98
	(UV-photo, 1.0 mg)	10	30	2	4	PLPEOOH	0.9	0.6	94
	(LOX-1, 86 mg)	10	30	12	4	PLPEOOH	80	65	98
PLPSOOMxP	(RB-photo, 24 mg)	10	30	6	4	PLPSOOH	22	14	98
	(UV-photo, 1.0 mg)	10	30	2	4	PLPSOOH	0.9	0.6	93
	(LOX-1, 83 mg)	10	30	12	4	PLPSOOH	78	60	98
ChLOOMxP	(RB-photo, 20 mg)	10	30	6	20	ChLOOH	18	11	98
	(UV-photo, 0.9 mg)	10	30	2	20	ChLOOH	0.8	0.5	95
	(LOX-1, 5.3 mg)	10	30	2	20	ChLOOH	4.8	2.3	95
LLLOOMxP	(RB-photo, 27 mg)	10	30	6	4	LLLOOH	26	19	95
	(UV-photo, 32 mg)	10	30	6	4	LLLOOH	30	21	98
	(LOX-1, 8.2 mg)	10	30	6	4	LLLOOH	7.6	5.0	98
LAOOMxP	(RB-photo, 24 mg)	5	30	1	4	LAOOH	22	14	95
	(UV-photo, 26 mg)	5	30	1	4	LAOOH	24	16	95
	(LOX-1, 85 mg)	5	30	6	4	LAOOH	81	57	98
LAMeOOMxP	(RB-photo, 30 mg)	5	30	1	4	LAMeOOH	28	19	95
	(UV-photo, 33 mg)	5	30	1	4	LAMeOOH	31	20	97
	(LOX-1, 93 mg)	5	30	6	4	LAMeOOH	90	78	>99

^aOxidation procedure.

^bLC retention times and MS profiles of hydroperoxides are as follows; PLPCOOH, 11.0 min, 790.6 [M + H]⁺; PLPEOOH, 10.5 min, 748.5 [M + H]⁺; PLPSOOH, 8.5 min, 790.4 [M − H]⁻; ChLOOH, 26.5 min, 703.6 [M + Na]⁺; LLLOOH, 23.0 min, 933.7 [M + Na]⁺; LAOOH, 15.5 min, 330.3 [M + NH₄]⁺; LAMeOOH, 11.5 min, 349.3 [M + Na]⁺.