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. Author manuscript; available in PMC: 2022 Apr 30.
Published in final edited form as: Biochem J. 2021 Apr 30;478(8):1571–1583. doi: 10.1042/BCJ20210138

Figure 1: Cell-based study of FUT8 enzymatic activity on high-mannose type glycoform of EPO and GM-CSF.

Figure 1:

a. MALDI-TOF MS of the N-glycans released from the EPO; b. MALDI-TOF MS of the N-glycans released from GM-CSF. The two glycoproteins were expressed in HEK 293T cells with the overexpression of FUT8 cultured in the presence of 1 μg/mL of kifunensine. Each spectrum was taken as a sum of ten random positions on the corresponding spot of the MALDI target plate.