Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 22;11:8698. doi: 10.1038/s41598-021-88103-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The visfatin localization in the porcine hypothalamus on days 10–12 of the estrous cycle. Immunoreactivity of visfatin was determined by fluorescent immunohistochemistry. Columns from left: first—visfatin expression, visualized by Alexa Fluor 555 as red fluorescence; second—nuclei stained with DAPI, visualized as blue fluorescence; third—merged images of channels (Olympus BX51, Olympus Soft Imaging Solutions, Germany). In the upper left corner of each image in the first column—negative control, where the primary antibody were replaced with non-immunosera. Particular images indicate the immunolocalization of visfatin in the diagonal band of Broca (DBB), supraoptic nucleus (SON), preoptic area (SDN), periventricular nucleus (PPN), lateral (LPA) and medial preoptic area (MPA), paraventricular nucleus (PVN), suprachiasmatic nucleus (SCN), arcuate nucleus (ARC), as well as ventromedial nucleus (VMH). Immunofluorescent staining was repeated on three pigs. Scale bar: 50 μm.

The visfatin localization in the porcine hypothalamus on days 10–12 of the estrous cycle. Immunoreactivity of visfatin was determined by fluorescent immunohistochemistry. Columns from left: first—visfatin expression, visualized by Alexa Fluor 555 as red fluorescence; second—nuclei stained with DAPI, visualized as blue fluorescence; third—merged images of channels (Olympus BX51, Olympus Soft Imaging Solutions, Germany). In the upper left corner of each image in the first column—negative control, where the primary antibody were replaced with non-immunosera. Particular images indicate the immunolocalization of visfatin in the diagonal band of Broca (DBB), supraoptic nucleus (SON), preoptic area (SDN), periventricular nucleus (PPN), lateral (LPA) and medial preoptic area (MPA), paraventricular nucleus (PVN), suprachiasmatic nucleus (SCN), arcuate nucleus (ARC), as well as ventromedial nucleus (VMH). Immunofluorescent staining was repeated on three pigs. Scale bar: 50 μm.