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. 2021 Apr 22;11:8698. doi: 10.1038/s41598-021-88103-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Visfatin gene and protein expression in the porcine preoptic area (POA) during the estrous cycle and early pregnancy. Gene and protein expression of visfatin in the porcine POA was determined during the estrous cycle on days: 2–3, 10–12, 14–16 and 17–19 (A,B), during early pregnancy on days: 10–11, 12–13, 15–16 and 27–28 (C,D) and compared between early pregnancy and days 10–12 of the estrous cycle (E,F). Gene expression was analyzed by qPCR. Protein expression was analyzed by Western blotting; upper panels: representative immunoblots; lower panels: densitometric analysis of visfatin protein relative to actin protein. Results are presented as means ± SEM (n = 5). Bars with different superscripts are significantly different (one-way ANOVA at p < 0.05 followed by Tuckey post hoc test at p < 0.05). Representative full length blots are attached as a Supplementary file 1.