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. 2021 Apr 22;11:8769. doi: 10.1038/s41598-021-88000-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Temporal course of HIF-1α signaling during AKI to CKD transition. (A) mRNA levels of Hif1a, (B) mRNA levels of Vegfa, (C) Protein levels of HIF-1α, including a representative cropped blot, and (D) Protein levels of VEGF, including a representative cropped blot. Data are represented as the mean ± SE. n = 4, for S, UNx, and IR + UNx groups, in each studied period: 30, 60, 90, and 120 days post-ischemia. White circles represent the S, gray squares represent the UNx and black triangles represent the IR + UNx groups. The one-way analysis of variance (ANOVA) was used to determine statistical differences, using the Bonferroni correction for multiple comparisons. *p < 0.05 versus S group and + p < 0.05 versus UNx group in their respective period. Full-length blots are presented in Supplementary Figs. 8 and 9.