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. 2021 Apr 22;11:8769. doi: 10.1038/s41598-021-88000-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bisulfite sequencing of two non-codifying upstream regions of Vegfa promoter for (A) the first-month and (B) the fourth month of follow-up for the Sham, UNx and IR + UNx groups, respectively. Arrows represented the forward and reverse primers for each amplicon. HIF-1α, p300, C/EBPα, and Nrf2 binding sites as is stated. White circles represented non-methylated CpG, and black circles represented methylated CpG, each circle represented an individual clone. Each C represented a cytosine in CpG context. TSS-Transcription star site; HRE-Hypoxia response element (ACGTG).