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. 2021 Apr 22;11:8690. doi: 10.1038/s41598-021-88099-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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AEA prevents the efflux of ethidium bromide (EtBr) from MDRSA CI-M. (A,B) Flow cytometry of EtBr retained in MDRSA CI-M 10 min (A) and 60 min (B) after treating the bacteria with different concentrations of AEA. (C) The summary of the geometric mean fluorescence intensity (MFI) of samples shown in (B). (D) The MFI of the various treatments in a time course study using flow cytometry. (E) A time course study measuring the fluorescence intensity (RFU) of EtBr using the Tecan plate reader. *p < 0.05.