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. 2021 Apr 22;11:8690. doi: 10.1038/s41598-021-88099-6

Figure 3.

Figure 3

(A) A kinetic study of the planktonic growth of MDRSA CI-M in the absence or presence of 50 μg/ml AEA and/or 50 μg/ml methicillin for 20 h. (B) A kinetic study of the planktonic growth of MDRSA CI-M in the absence or presence of 50 μg/ml AEA and/or 50 μg/ml norfloxacin for 20 h. (C) MDRSA CI-M at an OD of 0.1 was incubated in the presence of 50 μg/ml AEA for 4 h, then the AEA medium from half of the culture was replaced with fresh growth medium (washed), while the other half of the culture remained with AEA. The same procedure was done for control bacteria. This was followed by a kinetic study of the planktonic growth for 20 h. (D) The effect of AEA on the biofilm formation by the three S. aureus strains MSSA 25923, MSSA CI-G and MDRSA CI-M. After a 24 h incubation of the bacteria with increasing concentration of AEA, the metabolic activity of the formed biofilms was measured using the MTT assay. *p < 0.05; **p < 0.01.