Figure 4.

The effect of combined treatment of MDRSA CI-M with AEA and antibiotics on biofilm formation. (A) Biofilm formation after a 24 h incubation of MDRSA CI-M in the absence or presence of 25 or 50 μg/ml methicillin (MET) and increasing concentrations of AEA as measured by crystal violet staining. (B) Biofilm formation after a 24 h incubation of MDRSA CI-M in the absence or presence of 25 or 50 μg/ml norfloxacin (NOR) and increasing concentrations of AEA as measured by crystal violet staining. (C) MDRSA CI-M was allowed to form biofilms for 24 h. The biofilms were then exposed to 50 μg/ml methicillin (MET) and increasing concentrations of AEA for another 24 h and the metabolic activity of the remaining biofilms was measured using the MTT assay. *p < 0.05.