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. 2021 Apr 22;11:8690. doi: 10.1038/s41598-021-88099-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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AEA leads to a dose-dependent accumulation of DAPI. (A) Propidium iodide (PI) uptake of MDRSA CI-M treated with increasing concentrations of AEA for 2 h as determined by flow cytometry. (B) DAPI uptake of MDRSA CI-M treated with increasing concentrations of AEA for 2 h as determined by flow cytometry. (C) A summary of the geometric mean fluorescence intensity of DAPI accumulation in the AEA-treated bacteria presented in B. (D) The fluorescence intensity of nile red membrane staining of control and AEA-treated bacteria as determined by flow cytometry. (E) Spinning disk microscopy of control and 2 h AEA-treated MDRSA CI-M that were stained with nile red (red fluorescence) and DAPI (blue fluorescence). *p < 0.05.