Fig. 5. SsoPox W263I inhibits QS-regulated factors production of C. violaceum.

Mean levels of violacein (a) hydrogen cyanide (b), protease (c), and biofilm (d) production of C. violaceum untreated (purple) and treated cultures with 0.5 mg ml⁻¹ SsoPox W263I (yellow). Error bars represent the standard deviations of n = 3 biological replicates (each represented by one point). *P values <0.05; **P values <0.01 according to Student’s t test. Representative pictures of untreated (yellow squares) and lactonase treated cultures (yellow squares) (a) and their biofilm formation after coloration with crystal violet (d) are given. Network represents correlation multi-omics networks where proteins (squares) and metabolites (hexagons) involved in violacein (a) and hydrogen cyanide production (b) production and significantly impacted by SsoPox W263I are positively (green) or negatively (red) connected.