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. 2021 Apr 22;11:8798. doi: 10.1038/s41598-021-88302-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the enhancin gene (efp) (a), chitin-binding domain-containing protein gene (cbp) (b) and endo-α-N-acetylgalactosaminidase gene (eng) (c) regions of DAT561. Gray bars represent regions deleted in the mutants constructed in this study. Blue arrowheads represent primers used to confirm deletion of the target genes. (d) Detection of pMP19 and confirmation of the target gene deletions in the constructed mutants by PCR. The primers, enzyme and PCR conditions are listed in Supplementary Table S1. Except for DAT561, M. plutonius strains used in this study lost pMP19. In each gene deletion mutant, the target regions were deleted as expected.