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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Hepatology. 2021 May 14;73(6):2251–2265. doi: 10.1002/hep.31604

Figure 5. Loss of hepatocyte HNF4α aggravates HFCF diet-induced steatohepatitis.

Figure 5.

Hnf4αfl/fl mice and Hnf4αHep−/− mice were fed an HFCF diet for 20 weeks (n=8 per group). (A) Plasma ALT and AST levels. (B) Hepatic mRNA levels (left and right panels). (C) Hepatic proteins were analyzed by Western blot assays and then quantified (left panels). Hepatic cholesterol and BA levels were also analyzed (right panels). (D) Hepatic apoptosis was analyzed by TUNEL assays and then quantified (left panel). Hepatic protein levels were analyzed by Western blot assays and then quantified (middle and right panels). (E) Hepatic reactive oxygen species levels. (F) Hepatic sections were stained by Sirius Red (left panel), and hepatic hydroxyproline levels were quantified (right panel). *P<0.05, **P<0.01