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. 2021 Apr 22;12:2363. doi: 10.1038/s41467-021-22329-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a DLS analysis of crude extracts (blue) and SEC purified vesicles (green). Crude extract data are presented as mean intensity values for a given size ± standard deviation (SD) of n = 9 measurements (3 biologically independent extracts, each measured 3 times). Purified vesicle data are presented as mean intensity values for a given size ± SD of n = 3 measurements (one purified fraction measured 3 times). b NTA of purified vesicles collected from SEC. Mean and mode diameters observed in the particle size distribution are listed in the inset. Data are presented as mean ± standard error of the mean (SEM) of n = 5, 1 min NTA measurements of purified vesicles. c Illustration of particles detected in crude CFE extracts. d Cryo-EM micrographs of crude extracts. Black arrows indicate vesicles with apparent unilamellar morphology. White arrows indicate nested or multilamellar morphologies. Cropped images indicate representative vesicles. Scale bars are 100 nm. Uncropped images are available in Supplementary Fig. 3 and numbered with the corresponding cropped vesicles. e Cryo-EM micrographs of SEC purified vesicles. Cropped images indicate representative purified vesicle particles. Scale bars are 100 nm. Uncropped images are available in Supplementary Fig. 3 and numbered with the corresponding cropped vesicles. Micrographs are representative of three independent experiments. Source data for all panels are provided as a Source Data file.