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. 2021 Apr 22;12:2383. doi: 10.1038/s41467-021-22619-w

Fig. 3. Anti-tumor effect of HER3 kinase inhibition with CDX-3379 antibody: Disruption of HER3-p85 interaction.

Fig. 3

a HER3 co-IP analysis of signaling events in HNSCC cells treated by CDX-3379, a HER3 blocking antibody. Cal27 cells were treated by CDX-3379 (1 µg/ml) for 1 h and cell lysates were analyzed as indicated. b Cal27 cells and cells expressing PIK3CA H1047R were plated in 96-well ultra-low attachment culture plates at 100 cells per well (n = 20), and treated with PBS or CDX-3379 (1 μg/ml). The diameters of sphere colonies on each well were monitored using light microscopy. Shown are diameters (top) and representative photographs (bottom) of sphere colonies of each group. (Cal27 control, n = 20; Cal27 CDX-3379, n = 12; Cal27 PIK3CA H1047R control, n = 20; Cal27 PIK3CA H1047R CDX3379, n = 20;). Data were reported as mean ± SEM; two-sided Student’s t-test. c Western blot analysis of signaling events in HNSCC cells treated by CDX-3379. Wild-type and cells infected with PIK3CA H1047R (PIK3CA) of (left) Cal27, (middle) HN12, together with (right) Detroit 562 cells (harboring PIK3CA H1047R mutations) were serum starved overnight and treated by CDX-3379 at 100 ng/ml for 2 h. Cell lysates were analyzed as indicated. For both Fig. a and c, data shown are representative blots of results from three independent experiments with similar results. Source data are provided as a Source Data file.