Fig. 6.

Effect of FMT from KD- and LCD-fed mice on inflammatory responses and mucosal barrier function in colitis-induced germ-free mice. a Survival curves of DSS-induced colitis germ-free mice after FMT from mice fed with KD (FKD-C), LCD (FLCD-C), or ND (FND-C; control). b Body weight change in mice receiving 3% DSS in drinking water. c Representative colon images on 1 week after DSS induction and colon length shown as a chart. d Representative colon H&E staining images (original magnification ×200). Right: colonic section pathological scores. e DAI in each group. f Representative immunofluorescence, IHC and PAS staining images of colon tissues with antibodies against Occludin, ZO-1, and MUC-2 (original magnification ×200). g Colon Occludin and ZO-1 mRNA expression measured by qRT-PCR. h Number of PAS-positive cells per villus/crypt. *p < 0.05; **p < 0.01; ***p < 0.001. (FKD-C, n = 10; FLCD-C, n = 8; FND-C, n = 9; FND, n = 4). Results are expressed as mean ± SEM (b–e, g, h). KD ketogenic diet, LCD low-carbohydrate diet, ND normal diet, DSS dextran sulfate sodium, DAI disease activity index, IHC immunohistochemistry, PAS periodic acid schiff