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. 2021 Apr 22;11:8720. doi: 10.1038/s41598-021-88015-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TARDBP upstream sequences from the UCSC genome browser. The 721 nucleotides in the TARDBP upstream sequence include the predicted promoter region shown in a previous study. The red characters indicate the predicted i-motif region. Cytosine-rich regions are denoted by bolded text. The primer sequences used to generate the promoter/reporter constructs are underlined. Fwd forward primer, Rev reverse primer, TSS transcription start site. The UCSC genome browser shows 2 TSSs, and TSS1 is located 223 bp upstream of TSS2.