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. 2021 Apr 22;11:8758. doi: 10.1038/s41598-021-88129-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Simulation of the transcriptome-integrated CBM models (rMeCBMx) constructed using GIMME, E-Flux and HPCOF algorithms, (A) the number of non-zero (active) and zero (inactive) flux reactions from rMeCBM, rMeCBMx-GIMME-P25, rMeCBMx-GIMME-P75, rMeCBMx-EFlux, and rMeCBMx-HPCOF and (B) commonly predicted active reactions from all models, 236 of 468 total reactions denoted in the overlapped region of the Venn diagram and depicted in the schematic pathway as black solid arrows, postulated to be core metabolic reactions basically driving carbon assimilation in developing storage roots of cassava. Gray arrows denote trivial reactions obtained from some model predictions. The solid and dotted arrows represent genes associated with the reactions.