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. 2021 Apr 22;12:2382. doi: 10.1038/s41467-021-22599-x

Fig. 5. Characterization of in vitro biotinylation in the BirA*G3–ER mouse embryonic stem cell (mESC) line.

Fig. 5

a Western analysis of streptavidin binding to biotinylated proteins following 24 h of labeling at the indicated concentrations of biotin. All lanes are from the same blot (see Source Data for uncropped blots). b Quantification of biotin labeling relative to whole protein in a. c Western analysis of streptavidin binding to biotinylated proteins labeled in 50 μM biotin for the indicated times. All lanes are from the same blot (see Source Data for uncropped blots). d Quantification of biotin labeling relative to total protein in c. In this figure, representative results are shown from two western blots each. Source data are provided as a Source Data file.