Figure 2.

Estimation of total DNA and total RNA content during a proliferative cycle of P. falciparum blood stages. Synchronized cultures of DNA replicating (PfCRK4-DD on Shield-1) and non-DNA replicating (PfCRK4-DD off Shield-1; Ganter et al., 2017) parasites were treated with or without RNase. Subsequently, parasites were stained with SYBR green I, which labels both DNA and RNA (Theron et al., 2010; Ganter et al., 2017) and analyzed by flow cytometry. Total DNA and total RNA contents were estimated by calculating the difference in signal intensity with and without RNase treatment. Total DNA and total RNA contents were normalized by Min-Max scaling using the minimum and maximum values for DNA and RNA regardless of whether parasites replicate DNA or not.