Table 1.
The impact of heat treatment on leukocyte count, their differential composition, and the percentage of viable (non-necrotic) cells.
| Incubation temperature | 37°C | 41°C | ||||
|---|---|---|---|---|---|---|
| Incubation time | 1 h | 2 h | 4 h | 1 h | 2 h | 4 h |
| WBC cell/μl (×103) | 13.4, 1.8 | 12.9, 0.8 | 13.1, 1.0 | 13.7, 1.0 | 13.3, 1.1 | 12.8, 0.9 |
| Neutrophils % of WBC | 77.1, 0.6 | 76.8, 0.4 | 76.4, 0.2 | 76.5, 0.8 | 76.3, 0.4 | 69.6, 0.6 |
| Viable (PI−) cells (%) | 96.9, 1.4 | 95.1, 1.3 | 96.4, 1.7 | 95.2, 1.7 | 95.9, 1.7 | 94.6, 1.9 |
| Lymphocytes % of WBC | 19.2, 0.6 | 19.1, 0.8 | 19.8, 0.5 | 19.5, 0.4 | 19.3, 0.5 | 24.6, 0.9 |
| Viable (PI−) cells (%) | 95.8, 1.1 | 96.7, 1.7 | 96.6, 1.6 | 95.7, 1.3 | 94.0, 1.4 | 93.8, 1.7 |
| Monocytes % of WBC | 2.6, 0.3 | 2.5, 0.3 | 2.4, 0.2 | 2.4, 0.1 | 2.6, 0.2 | 3.1, 0.3 |
| Viable (PI−) cells (%) | 96.7, 1.1 | 95.8, 1.1 | 95.5, 1.3 | 95.5, 1.2 | 96.4, 1.3 | 94.0, 1.4 |
Microscopic estimation of the total leukocyte count (WBC) was performed using Neubauer counting chamber after incubation of blood with Türk solution for erythrolysis. PI, propidium iodide.