Figure 4.

Deletion of forJ results in increased transcription of for genes and deletion of forGF decreases expression

In accordance with observed titers, deletion of forJ results in an increase in the expression of all other for cluster transcripts. Deletion of forGF results in a decrease in expression of all biosynthetic transcripts but an increase in the expression of the repressor gene forJ, thereby inhibiting biosynthesis. In the forZ deletion mutant, expression of the resistance transporter forAA is decreased, suggesting forZ is required to activate its transcription. There is a small decrease in the levels of other transcripts in the forZ mutant, suggesting this regulator may also indirectly influence expression of these genes without binding to their promoters. Error bars represent SD across three biological and two technical experimental replicates.