Table 1.
Fasamycin and formicamycin production by engineered S. formicae strains on solid soya flour mannitol agar and in liquid soya flour mannitol
| Strain | Fasamycins titer (μM) |
Formicamycins titer (μM) |
Combined titer (μM) |
Fasamycins titer (μM) |
Formicamycins titer (μM) |
Combined titer (μM) |
|---|---|---|---|---|---|---|
| Solid | Liquid | |||||
| Wild-type | 6.5 ± 9.6 | 81.3 ± 21.6 | 87.9 ± 74.2 | 0 | 0 | 0 |
| Wild-type + forJ | 0 | 24.8 ± 4.7 | 24.8 ± 4.7 | 0 | 0 | 0 |
| ΔforJ | 186.2 ± 22.2 | 406.5 ± 42.1 | 592.7 ± 69.4 | 6 ± 0.2 | 624.5 ± 29.4 | 630.5.4 ± 29.6 |
| ΔforJ + forJa | 170.8 ± 15.4 | 558.8 ± 53.5 | 729.6 ± 74.8 | 2.7 ± 0.6 | 657.3 ± 40.7 | 660 ± 41.3 |
| ΔforJ + forJb | 78.0 ± 60.2 | 784.0 ± 18.9 | 862.0 ± 36.6 | 210 ± 36.0 | 423.1 ± 72.4 | 633.1 ± 138.1 |
| Wild-type + forGF | 8.6 ± 0.02 | 155.7 ± 18.7 | 164.3 ± 23.7 | Not tested | not tested | not tested |
| ΔforGF | 0 | 0 | 0 | not tested | not tested | not tested |
| ΔforGF + forGF | 13.1 ± 6.7 | 153.7 ± 73.6 | 165.7 ± 86.1 | not tested | not tested | not tested |
| ΔforJ + forGF | 56.7 ± 52.9 | 814.2 ± 139.8 | 873.4 ± 195.9 | 275.4 ± 11.6 | 759.8 ± 193.8 | 1035.2 ± 212.6 |
| ΔforJ ΔforGF | 76.3 ± 36.8 | 648.1 ± 112.7 | 724.5 ± 147.8 | 242.9 ± 21.6 | 355.3 ± 82.1 | 598.1 ± 85.7 |
| ΔforJ ΔforZ | 38.6 ± 31.2 | 388.5 ± 24.8 | 427.1 ± 31.6 | 516.48 ± 297.4 | 409.1 ± 95.4 | 925.6 ± 341.2 |
| ΔforZ | 11.1 ± 10.6 | 49.8 ± 3.7 | 60.9 ± 19.7 | not tested | not tested | not tested |
| ΔforZ + forZ | 13.6 ± 15.8 | 21.0 ± 15.7 | 31.8 ± 38 | not tested | not tested | not tested |
| ΔforV | 72.8 ± 60.3 | 0 | 72.8 ± 60.3 | 1.3 ± 1.8 | 0 | 1.3 ± 1.8 |
| ΔforJΔforV | 587.5 ± 268.1 | 0 | 587.5 ± 268.1 | 79.4 ± 8.1 | 0 | 79.4 ± 8.1 |
| ΔforX | 42.3 ± 22.6 | 0 | 42.3 ± 22.6 | 2.54 ± 4.6 | 0 | 2.54 ± 4.6 |
| ΔforJΔforX | 782.6 ± 147.8 | 0 | 782.6 ± 147.8 | 274.3 ± 325.4 | 0 | 274.3 ± 325.4 |
Values are mean ± SD; wild-type, n = 16; mutants, n = 3.
a
forJ under control of the native promoter.
b
forJ under control of the ermE∗ promoter.