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. 2021 Feb 10;6(1):ysab008. doi: 10.1093/synbio/ysab008

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© The Author(s) 2021. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — sgRNA targets for sADH (CAETHG_0553), TetR (CAETHG_0459 and BudA (CAETHG_2932). Distance in base pairs (bp) between the first bp of the start codon and the PAM site is listed on the top of the guide. On-target activity scores are provided below each guide. Guides IDs numerically assigned with the most 5ʹ as ‘CAETHG_XXX-Guide-1’.