Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 10;6(1):ysab008. doi: 10.1093/synbio/ysab008

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — Analysis of TetR (CAETHG_0459) KD effect on expression using RT-qPCR. Copy number (C#) ratio of TetR to 16S from KD (guide-2 (+79), blue) and negative control (CAETHG_0533 guide-4, green). Data represented as points are average of technical replicates, where error bars represent standard deviation. The points are grouped by biological replicate (N = 3) and split into 48 and 120 h sampling points (light color/left and dark color/right, respectively). Bars represent means of data and are associated to their respective points if colored, while black bars are associated to KD and control (i.e. both timepoints). The difference between the means of KD and control samples was shown to be statistically significant using various methods, represented as P < 0.05 here (see Supplementary Text for various methods’ P-values).