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. 2021 Apr 9;12:616050. doi: 10.3389/fmicb.2021.616050

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Copyright © 2021 Bednarz, Millan-Oropeza, Kotowska, Świat, Quispe Haro, Henry and Pawlik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypes of S. coelicolor A3(2) wild-type (M145) and mutant strains cultivated on solid medium 79NG. CPK, RED, and ACT are visible as yellow, red and blue pigments, respectively. Aerial mycelium is white. (A) Streptomyces strains grown as spots; comp., complemented strains; scoT_OE, overexpression of scoT; φ, strains with an empty plasmid. (B) Growth on plates covered with cellophane disks and biomass collected for proteomic analysis (in Eppendorf tubes). (C) Assay for calcium dependent antibiotic production. Inhibition zone around the colony of ΔcpkO strain in the presence of Ca(NO₃)₂ indicates CDA production.