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. 2021 Apr 9;15:662578. doi: 10.3389/fncel.2021.662578

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Copyright © 2021 Tavčar, Potokar, Kolenc, Korva, Avšič-Županc, Zorec and Jorgačevski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transmission electron micrographs of selected neurotropic viruses. (A) SARS-CoV-2 (marked by arrowhead) isolated on Vero E6 cells, ultracentrifuged onto formvar coated with carbon stabilized grids and negatively stained by using 2% phosphotungstic acid. (B) West Nile viruses (marked by arrowheads) in an infected SK-N-SH cell; the image shows an Epon embedded ultrathin section contrasted with uranyl acetate and lead citrate. (C) Zika virus (see arrowheads, strain Uganda 976) in an infected Vero E6 cell; the image shows an Epon embedded ultrathin section contrasted with uranyl acetate and lead citrate. Strings of viruses represent virus localization within endoplasmic reticulum. (D) Tick-borne encephalitis virus (marked by arrowheads) isolated on Vero E6 cells, ultracentrifuged onto formvar coated with carbon stabilized grids and negatively stained using 2% phosphotungstic acid. Electron micrographs were obtained by transmission electron microscope (JEM-1400 Plus, JEOL, Tokyo, Japan) at 120 kV.