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. 2021 Apr 9;15:660561. doi: 10.3389/fncel.2021.660561

FIGURE 2.

FIGURE 2

ARC39 increases frequency and amplitude of EPSCs in CA1 pyramidal cells. Spontaneously occurring excitatory postsynaptic events were recorded in the whole-cell voltage-clamp configuration at –70 mV in PTX (100 μM). Spontaneous EPSCs (spEPSC) and miniature EPSCs (mEPSCs) were collected in the absence and presence of TTX (0.5 μM), respectively. (A) Raw traces of spEPSCs from a wt cell (black trace) and an ASM-KO cell (gray trace) were collected in aCSF with normal K+ concentration (3 mM). (B,C) spIPSCs in ASM-KO pyramidal cells were more frequent and larger in amplitude in both solutions with normal K+ (3 mM) and with elevated K+ concentration (6 mM, to enhance synaptic events). (D) Raw traces of spEPSCs from a wt cell were collected in solution with high K+ (6 mM) before ARC39 application (control), 6 min in ARC39 (1 μM) and after 10 min of wash. Superimposed traces represent averaged events from same cell before (black trace) and during drug application (red trace). (E–G) Histograms summarize effects of acutely applied ARC39 (1 μM) on frequency (E) and averaged amplitude (F) of spEPSCs and mEPSCs in wt pyramidal cells, and concomitant shift of holding current (G). Insets above in panels (E,F) are the cumulative probability plots for inter-event interval (reciprocal of frequency) and peak amplitude. *p < 0.05, **p < 0.01, and ***p < 0.001.