Figure 3. Effect of RyR2 mutations on caffeine-induced Ca²⁺ release in HEK293 cells.

HEK293 cells were transfected with RyR2 WT or mutants. Fluorescence intensity of the fluo-3-loaded transfected cells was monitored continuously before and after each caffeine addition. The numbers (under the traces) indicate caffeine concentrations. Traces shown are from representative experiments (n=3). (A) RyR-WT. (B-Q) RyR2 mutants tested in the caffeine-induced Ca²⁺ release experiments.