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. 2021 Apr 9;11:639980. doi: 10.3389/fonc.2021.639980

Figure 4.

Figure 4

The function of LIPE-AS1/miR-195-5p in cervical cancer cells. (A) MiR-195-5p mimics and inhibitors were taken to construct miR-195-5p over- and down-expressing cell lines, respectively, and further transfected with LIPE-AS1 over-expressed plasmids or sh-LIPE-AS1 to perform compensation experiments. CCK8 method (B) and BrdU method (C) were employed to detect HeLa and HCC94 cell proliferation. (D) Flow cytometry was performed to test the apoptosis of HeLa and HCC94 cells. (E) Transwell method was carried out to examine HeLa and HCC94 cell invasion. The expressions of E-cadherin, Snail, and Vimentin in HeLa (F) and HCC94 cells (G) were examined by Western blot. **P < 0.01, ***P < 0.001 vs. Vector or sh-NC group. &P < 0.05, &&P < 0.01, &&&P < 0.001 vs. LIPE-AS1 group or sh-LIPE -AS1 group. N = 3.