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. 2021 Apr 9;11:639980. doi: 10.3389/fonc.2021.639980

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Copyright © 2021 Zhang, Jiang, Wang, Cheng and Fu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of LIPE-AS1/miR-195-5p on the MAPK/ERK pathway. LIPE-AS1 over- and low-expressing cell models were constructed in HeLa (A) and HCC94 cells (B), respectively. Western blot was used to measure MAP3K8 and ERK protein expression in the cells. (C,D) Transfection of cervical cancer cells with miR-195-5p mimics and inhibitors, LIPE-AS1 over-expressed plasmid or sh-LIPE-AS1. Western blot was taken to measure the protein expressions of MAP3K8 and ERK in cells. (E,F) Tissue immunofluorescence was carried out to detect MAP3K8 in the tumor tissues (the grouping was the same as Figure 5). *P < 0.05, **P < 0.01, ***P < 0.001. N = 3.