FIGURE 1.

(A) CHO DP-12 cells were grown under standard conditions until cells entered stationary phase (day 5) then process parameters were changed as shown followed by cell harvest and supernatant collection after 48 h. (B) Schematic overview of analysis following cell harvest. Bioprocess monitoring was carried out on a daily basis to assess viable cell density, metabolite or nutrient concentration and IgG production rate. Following batch culture, cells were lysed, and samples were prepared for quantitative proteomic profiling applying TMT labeling as indicated. Complementary product characterization was done to detect sequence variants, modifications, and structural changes.